Inside our Lab

Our Lab is full of passionate people dedicated to bring you the most advanced technology in DNA Testing. With 18 years of experience in the field of DNA testing, our state-of-the-art laboratory is certified by the highest standards including CLIA, ISO 15189, ISO 9001, DNA Profiling External Quality Assessment.

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DNA Analysis

The test procedure begins once your saliva sample arrives at our laboratory. The first step is DNA isolation and detection. Your samples will be transferred to the skilled hands of our DNA technicians, who follow a carefully optimized protocol of DNA isolation, DNA amplification, and DNA detection.

 

During the detection reaction, we extract the information hidden in your genes. The final step is DNA sequence data interpretation, wherein our highly qualified DNA experts capitalize on their decades of DNA research from leading institutions around the world.

 

Using the latest advances in the field of bioinformatics, our DNA experts will prepare a detailed report indicating the presence of any SNPs and any potential risk for disease.

PCR and DNA Fragmentation

The next step is critical for a successful detection of SNP. During this step, the fragmented DNA mixture from the previous step is transferred to the chip, on which short single-stranded DNA molecules called primers are chemically attached. A reaction mixture contains nucleotides for DNA synthesis as well as the four DNA terminator nucleotides labelled with fluorescent dyes – each nucleotide with a distinct color.

 

When a terminator nucleotide is incorporated into the DNA molecule, the synthesis of this particular molecule stops. By the end of this reaction, an array of DNA fragments of different sizes each labelled with a unique color depending on the terminator nucleotide is generated. This reaction is carefully optimized to obtain the highest signal-to-noise ratio and to ensure the specificity of detection.

APEX Reaction

The next step is critical for a successful detection of SNP. During this step, the fragmented DNA mixture from the previous step is transferred to the chip, on which short single-stranded DNA molecules called primers are chemically attached. A reaction mixture contains nucleotides for DNA synthesis as well as the four DNA terminator nucleotides labelled with fluorescent dyes – each nucleotide with a distinct color.

 

When a terminator nucleotide is incorporated into the DNA molecule, the synthesis of this particular molecule stops. By the end of this reaction, an array of DNA fragments of different sizes each labelled with a unique color depending on the terminator nucleotide is generated. This reaction is carefully optimized to obtain the highest signal-to-noise ratio and to ensure the specificity of detection.

APEX Detection

For SNP detection, the chips with fluorescently labeled DNA arrays are visualized in a QuattroImager, a device equipped with four lasers and a highly sensitive, specialized camera. The fluorescent color patterns are recorded and then analyzed by the Genorama Genotyping Software, which converts the image information into DNA sequence data. This sequence data is then used for SNP detection.

Data Analysis and Interpretation

Using the DNA sequence data, the DNA expert, in collaboration with bioinformaticians, determines the presence of SNPs in the DNA regions related to disease. Comparing this information with previous knowledge, the DNA expert then computes the risk of acquiring a particular disease. A detailed analysis containing sequencing information and the presence of any SNPs is delivered to the client.

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